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Detection of pathogens in different matrixes with appropriate reliable tools

Extraction of nematods from soil samples
Bacterial analysis of water samples using filtration
Real time PCR test

There is not usually only one effective method of fighting against pathogens (for example bacteria and nematodes); control is often based on a set of preventive measures to avoid the emergence and spread of pathogens.

To this end, the health status of the biological material produced and/or introduced in the territory needs to be checked along with the health status of the environment, which could be a reservoir for some pathogens. This includes water, soil, weeds, mud, and industrial byproducts. Detection tools must be reliable, and also suited to each of the substrates to be tested.

  • The main tools under study for the plant component are:
    • PVY detection by real-time RT-PCR applied on different potato organs;
    • Detection of “root-knot nematodes” directly on the plant without the need for prior isolation of the pathogen.
  • Methods will be developed for the detection of different pathogens (bacteria, fungi, nematodes) for use in more complex matrices including effluents from food processing plants, sludges, green wastes, by:
    • Using the biological properties of the target microorganisms (trapping by host plants, isolation on selective media, etc.) combined (or not) with molecular characterization,
    • Developing reliable methods of extracting DNA directly from complex matrices.
Nematode extraction by elutriation

Nematode extraction by elutriation 

pectinolytic bacteria

Isolation of pectinolytic bacteria with semi-selective medium (CVP) 

nematode larvae

NEMATOOLS : a CASDAR (French Ministry of Agriculture) project on nematodes of major importance for potato production

Among the plant-parasitic nematodes, Meloidogyne chitwoodi, M. fallax, Globodera pallida and G. rostochiensis are the most detrimental on potato....
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