Know more

Our use of cookies

Cookies are a set of data stored on a user’s device when the user browses a web site. The data is in a file containing an ID number, the name of the server which deposited it and, in some cases, an expiry date. We use cookies to record information about your visit, language of preference, and other parameters on the site in order to optimise your next visit and make the site even more useful to you.

To improve your experience, we use cookies to store certain browsing information and provide secure navigation, and to collect statistics with a view to improve the site’s features. For a complete list of the cookies we use, download “Ghostery”, a free plug-in for browsers which can detect, and, in some cases, block cookies.

Ghostery is available here for free: https://www.ghostery.com/fr/products/

You can also visit the CNIL web site for instructions on how to configure your browser to manage cookie storage on your device.

In the case of third-party advertising cookies, you can also visit the following site: http://www.youronlinechoices.com/fr/controler-ses-cookies/, offered by digital advertising professionals within the European Digital Advertising Alliance (EDAA). From the site, you can deny or accept the cookies used by advertising professionals who are members.

It is also possible to block certain third-party cookies directly via publishers:

Cookie type

Means of blocking

Analytical and performance cookies

Realytics
Google Analytics
Spoteffects
Optimizely

Targeted advertising cookies

DoubleClick
Mediarithmics

The following types of cookies may be used on our websites:

Mandatory cookies

Functional cookies

Social media and advertising cookies

These cookies are needed to ensure the proper functioning of the site and cannot be disabled. They help ensure a secure connection and the basic availability of our website.

These cookies allow us to analyse site use in order to measure and optimise performance. They allow us to store your sign-in information and display the different components of our website in a more coherent way.

These cookies are used by advertising agencies such as Google and by social media sites such as LinkedIn and Facebook. Among other things, they allow pages to be shared on social media, the posting of comments, and the publication (on our site or elsewhere) of ads that reflect your centres of interest.

Our EZPublish content management system (CMS) uses CAS and PHP session cookies and the New Relic cookie for monitoring purposes (IP, response times).

These cookies are deleted at the end of the browsing session (when you log off or close your browser window)

Our EZPublish content management system (CMS) uses the XiTi cookie to measure traffic. Our service provider is AT Internet. This company stores data (IPs, date and time of access, length of the visit and pages viewed) for six months.

Our EZPublish content management system (CMS) does not use this type of cookie.

For more information about the cookies we use, contact INRA’s Data Protection Officer by email at cil-dpo@inra.fr or by post at:

INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu INRA logo Inra FN3PT Fédération Nationale des Producteurs de Plant de Pomme de Terre

Home page

Detection of pathogens in different matrixes with appropriate reliable tools

Extraction of nematods from soil samples
Bacterial analysis of water samples using filtration
Real time PCR test

There is not usually only one effective method of fighting against pathogens (for example bacteria and nematodes); control is often based on a set of preventive measures to avoid the emergence and spread of pathogens.

To this end, the health status of the biological material produced and/or introduced in the territory needs to be checked along with the health status of the environment, which could be a reservoir for some pathogens. This includes water, soil, weeds, mud, and industrial byproducts. Detection tools must be reliable, and also suited to each of the substrates to be tested.

  • The main tools under study for the plant component are:
    • PVY detection by real-time RT-PCR applied on different potato organs;
    • Detection of “root-knot nematodes” directly on the plant without the need for prior isolation of the pathogen.
  • Methods will be developed for the detection of different pathogens (bacteria, fungi, nematodes) for use in more complex matrices including effluents from food processing plants, sludges, green wastes, by:
    • Using the biological properties of the target microorganisms (trapping by host plants, isolation on selective media, etc.) combined (or not) with molecular characterization,
    • Developing reliable methods of extracting DNA directly from complex matrices.
Nematode extraction by elutriation

Nematode extraction by elutriation 

pectinolytic bacteria

Isolation of pectinolytic bacteria with semi-selective medium (CVP) 

nematode larvae

NEMATOOLS : a CASDAR (French Ministry of Agriculture) project on nematodes of major importance for potato production

Among the plant-parasitic nematodes, Meloidogyne chitwoodi, M. fallax, Globodera pallida and G. rostochiensis are the most detrimental on potato....
Read more