There is not usually only one effective method of fighting against pathogens (for example bacteria and nematodes); control is often based on a set of preventive measures to avoid the emergence and spread of pathogens.
To this end, the health status of the biological material produced and/or introduced in the territory needs to be checked along with the health status of the environment, which could be a reservoir for some pathogens. This includes water, soil, weeds, mud, and industrial byproducts. Detection tools must be reliable, and also suited to each of the substrates to be tested.
- The main tools under study for the plant component are:
- PVY detection by real-time RT-PCR applied on different potato organs;
- Detection of “root-knot nematodes” directly on the plant without the need for prior isolation of the pathogen.
- Methods will be developed for the detection of different pathogens (bacteria, fungi, nematodes) for use in more complex matrices including effluents from food processing plants, sludges, green wastes, by:
- Using the biological properties of the target microorganisms (trapping by host plants, isolation on selective media, etc.) combined (or not) with molecular characterization,
- Developing reliable methods of extracting DNA directly from complex matrices.